Year: 2026 | Month: May | Volume: 16 | Issue: 5 | Pages: 30-38
DOI: https://doi.org/10.52403/ijhsr.20260504
Multidrug-Resistance Profile of Non-Fermenting Gram Negative Bacilli Isolated from Various Clinical Samples Received in a Tertiary Care Hospital, Ballari - Cross Sectional Study
Nikitha M1, Surekha Y A2, Mariraj Jeer3, Arun T L4
1,4PG student, Department of Microbiology, BMCRC, Ballari.
2Professor, Department of Microbiology, BMCRC, Ballari.
3HOD, Department of Microbiology, BMCRC, Ballari.
Corresponding Author: Dr. Arun T L
ABSTRACT
Background: Non-fermenting Gram-negative bacilli (NF-GNB) are important causes of healthcare-associated infections and are increasingly associated with multidrug resistance, posing serious therapeutic challenges.
Objectives: To determine the prevalence of NF-GNB among culture-positive clinical samples and to assess their antimicrobial susceptibility patterns in a tertiary care hospital.
Materials and Methods: A cross-sectional study was conducted over six months (July–December 2025) in a tertiary care hospital laboratory. A total of 388 clinical specimens were processed using standard microbiological techniques. Identification of NF-GNB was performed by conventional biochemical methods. Antimicrobial susceptibility testing was carried out using the Kirby–Bauer disc diffusion method, and results were interpreted according to CLSI guidelines. Multidrug resistance was defined as resistance to three or more antibiotic classes.
Results: Of 223 culture-positive samples, 50 NF-GNB were isolated, of which 25 (50%) were multidrug resistant. Acinetobacter species predominated (76%), followed by Pseudomonas aeruginosa (16%). Blood samples were the major source (44%). High resistance was observed to gentamicin (76%), ciprofloxacin (80%), and imipenem (60%), while moderate susceptibility was noted for meropenem (40%), tigecycline (32%), and cotrimoxazole (32%).
Conclusion: The study demonstrates a high burden of MDR NF-GNB, emphasizing the need for regular surveillance, antimicrobial stewardship, and strict infection-control measures.
Key words: Non-fermenting Gram-negative bacilli; Multidrug resistance; Acinetobacter; Pseudomonas aeruginosa; Antimicrobial susceptibility; Healthcare-associated infections; Kirby–Bauer disc diffusion.