Year: 2026 | Month: April | Volume: 16 | Issue: 4 | Pages: 183-199
DOI: https://doi.org/10.52403/ijhsr.20260423
Cumulative Antibiogram and Antimicrobial Susceptibility Patterns of Pus Isolates Using WHONET 5.6 2025 in a Microbiology Department of Clinical Laboratory in Navsari, South Gujarat
Manoj A Kahar1, PrashantKumar Jagubhai Patel2, Dhvani Y Kahar3, Nafisa Patel4
1Lab Director, Bhanumati Clinical Laboratory Navsari, Gujarat, India.
2Assistant Professor, Department of Pathology, Kiran Medical College, Surat, Gujarat, India.
3Student of M.Sc. in Microbiology, Naran Lala College of Professional & Applied Sciences, Navsari, Gujarat, India.
4Associate Professor, Department of Microbiology, Naran Lala College of Professional & Applied Sciences, Navsari, Gujarat, India.
Corresponding Author: Manoj A Kahar
ABSTRACT
Background: Pyogenic infections leading to pus formation are common in clinical practice. Increasing Antimicrobial Resistance (AMR) poses a major therapeutic challenge. Local cumulative antibiograms are essential for guiding empirical therapy.
Aims: To identify etiologies of pyogenic infections for 4 years (January 2022 to December1 2025) & to identify susceptibility pattern of pathogens isolated. To familiarize with WHONET software 2025 version 5.6 for preparing Antibiogram & to generate an Antibiogram for pus isolates which can guide empirical treatment.
Materials & Methods: A retrospective observational study was conducted analysing non-duplicate pus isolates from January 2022 to December 2025. Antimicrobial susceptibility data were processed using WHONET software 2025 version 5.6 following Analysis and Presentation of Cumulative Antimicrobial Susceptibility Test Data Clinical & Laboratory Standard Institute Analysis (CLSI) M39 5th edition guidelines. Findings of present study were compared with published national & international studies.
Results: 264 out of 517 pus samples resulted in positive isolates giving a yield of 51.06% with 6 species being isolated; Gram negative bacteria were 184 (69.69%) & Gram positive bacteria were 80 (30.30%). The most common organism isolated was Staphylococcus aureus 80 (30.30%) followed by Escherichia coli 42 (15.90%). The least isolated organism was Enterobacter spp. 12.12%. The most sensitive antimicrobial for both Gram negative & Gram positive was Trimethopirm-sulfamethoxazole (Cotrimoxazole) & the susceptibility was for E. coli 73.7%, for Enterobacter spp. 90.9%, for Klebsiella pneumoniae 70%, for Klebsiella spp. 81.8%, & Staphylococcus aureus 95.8%.
Conclusion: This study confirms that the bacteria implicated in pyogenic infection forming pus are S. aureus, E. coli, K. pneumoniae, Klebsiella spp., P. aeruginosa, Enterococcus spp. These organisms have developed drug resistance to some important antimicrobials such as cefazolin, cephalosporins & fluoroquinolones. Appropriate & rational selection of antimicrobials would limit the emerging drug resistance in the future with the use of local antibiograms.
Key words: Antimicrobial resistance, WHONET, cumulative antibiogram, pus, CLSI M39.