Year: 2025 | Month: July | Volume: 15 | Issue: 7 | Pages: 324-338
DOI: https://doi.org/10.52403/ijhsr.20250739
A Rapid and Precise Technique for Identifying 5-HIAA in Complex Biological Specimens
Vrushali Bhalchim1, Vaishali Undale2
1Assistant Professor, Department of Pharmacology, Amity Institute of Pharmacy, Amity University Maharashtra, Mumbai-410206, Maharashtra, India
2Associate Professor, Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pune, Maharashtra, India
Corresponding Author: Dr. Vrushali Bhalchim
ABSTRACT
Ehrlich reagent has been commonly used as colorimetric reagents for detection of indole group compound. The method is advancement for the colorimetric determination of 5-HIAA metabolite. The preparation of reaction mixture containing Ehrlich reagent (100 mg) in Conc HCl: Methanol (9:1) is simple, requires less time consuming. The sample is pre-treated with 0.1M per chloric acid for deproteinization of protein or interfering components is removed and the filtrate obtained after treatment of a small quantity of urine. The addition of Ehrlich's reagent mixture to this filtrate results in the immediate production of a stable green colour within 10sec of interaction of 5-HIAA present in urine sample. The determination is accurate, very sensitive and rapid, and reaction is completed within 1 min.
Key words: 5-HIAA, Ehrlich reagent, Colorimetric detection.