Year: 2025 | Month: December | Volume: 15 | Issue: 12 | Pages: 83-92
DOI: https://doi.org/10.52403/ijhsr.20251211
Isolation and Prevalence of Multi-Drug Resistant Acinetobacter from Various Clinical Samples in a Tertiary Care Hospital
Qursheed Sultana1, R. Saraswati Jayanthi2, K. Pavani3
1,2Professor, 3Professor & HOD
Department of Microbiology, RVM Institute of Medical Sciences and Research Centre, Mulugu, Telangana
Corresponding Author: Qursheed Sultana
ABSTRACT
BACKGROUND: Acinetobacter species are emerging pathogens in healthcare settings, responsible for many infections, including bacteraemia, pneumonia, meningitis, peritonitis and wound infections. Their ability to develop multidrug resistance through various resistance determinants poses significant challenges for Treatment. Timely detection and reporting of these multidrug-resistant pathogens would be useful not only for guiding an appropriate antibiotic therapy but also in controlling the spread of these drug-resistant strains in the hospital.
OBJECTIVES:
1)To isolate and study the prevalence of Acinetobacter species from all clinical samples received in the microbiology lab from inpatient wards and ICUS OF a tertiary care hospital for a period of one year.
2)To determine the Antibiotic resistance pattern of Acinetobacter species.
3) To determine the prevalence of multidrug-resistant and carbapenem-resistant Acinetobacter species.
MATERIALS AND METHODS: A retrospective observational study was conducted in the microbiology department of RVMIMS&RC, MULUGU MANDAL of Siddipet district for a period of one year.
Samples were taken from patients admitted to different ICUs and wards of the hospital and processed according to the standard microbiological procedures. Identification and antibiotic susceptibility testing were done in a fully automated Vitek 2 system. All clinical samples and patients of all age groups were included, and duplicate isolates from the same patient were excluded from the study.
RESULTS: During this study, a total of 7855 samples were processed and 1171(14.90%). samples were culture positive, out of which 136 (11.61%) were Acinetobacter species, and Acinetobacter baumannii (92.6%) was the commonest of Acinetobacter species. Acinetobacter baumannii was the most prevalent organism in ICUS (58.08%), affecting elderly patients above 60 years (40.44%) and most commonly affecting Males (74.26%). Acinetobacter baumannii was most commonly isolated from pus samples (36.03%), and they were resistant to commonly used drugs. They were multidrug-resistant and carbapenem-resistant. Colistin was 100% sensitive, although it was not confirmed by the MBC method. Good sensitivity was seen to the combination drug, like Ampicillin-sulbactam (57.57%) and 54% to minocycline.
CONCLUSION: The present study showed the alarming trends of resistance of Acinetobacter strains isolated from clinical specimens to the various classes of antimicrobials. The improvement of microbiological techniques for quick and more accurate identification of bacteria is necessary for the selection of appropriate treatments.
Key words: Acinetobacter, Multidrug resistant, Carbapenem resistant