Year: 2024 | Month: April | Volume: 14 | Issue: 4 | Pages: 542-549
DOI: https://doi.org/10.52403/ijhsr.20240469
Formation of Biofilms at Surgical Site Infection Caused by Pseudomonas aeruginosa and Their Clinical Significance
Aditi Patil1, Deepashri Naik2
1MSc. Resident, Department of Microbiology, MGM Medical College, Kamothe, Navi Mumbai Maharashtra, India.
2Associate Professor, Department of Microbiology, MGM Medical College, Kamothe, Navi Mumbai Maharashtra, India.
Corresponding Author: Dr Deepashri Naik
ABSTRACT
Introduction: Pseudomonas aeruginosa is one of the primary opportunistic pathogens that causes a variety of nosocomial infections.1 The ability of P. aeruginosa to form biofilms in a lot of environments renders antibiotic treatment unproductive and promotes persistent infectious diseases.2 This is a Descriptive and Prospective study to identify formation of biofilms on Surgical sites and to evaluate its effects and significance.
Materials & Methods: All swabs from surgical sites were collected from the period February 2021 to January 2022 and were subjected to Standard Microbiological procedures like Gram staining and various biochemical reactions to identify Pseudomonas aeruginosa. Antibiotic Susceptibility Test was performed by Kirby-Bauer Disk Diffusion Method. Further, biofilm production was investigated from the isolated Pseudomonas aeruginosa by Congo Red Agar method and Tissue Culture Plate method. Conclusion was derived by taking Co-morbidities into consideration too.
Results: Out of 500 samples, 475 samples were sterile and 5 samples showed growth of Pseudomonas aeruginosa. These 5 samples when tested for biofilm production by Congo Red Agar method, 2 of them were identified as biofilm producers while by Spectrophotometric method (Tissue Culture Plate method) 3 isolates were identified as biofilm producers (2 strong and 1 weak).
Conclusion: Hence, we can conclude that 60% of Pseudomonas aeruginosa can form biofilms at Surgical Site Infection. The biofilm producers also show increased resistance to antibiotics by almost 80%, causing a persistent infection and delay the wound healing of surgical site specially in patients with co- morbidities.
Key words: Pseudomonas aeruginosa, Biofilm, Pus, Surgical site Infections, Kirby-Bauer Disk Diffusion Method.