Original Research Article
Year: 2023 | Month: June | Volume: 13 | Issue: 6 | Pages: 148-152
DOI: https://doi.org/10.52403/ijhsr.20230626
Assessment of Ocimum sanctum Extracts as an Antifungal Agent against Aspergillus brasiliensis
Harshita Sisodia1, Pravina Rathore2
1,2Department of Botany
1,2DBhupal Nobles’ University, Udaipur (Rajasthan), India
Corresponding Author: Harshita Sisodia
ABSTRACT
In many regions across the globe, there exists compelling evidence of the historical use of medicinal plants to combat diseases caused by diverse pathogenic microorganisms. Traditional therapeutic practices have relied on specific plant species known for their antimicrobial properties, which were engaged in therapeutic treatments. These plants possess a multitude of biological compounds that hold potential for the development of new drugs aimed at enhancing human well-being. A large proportion of the population in developing countries still relies on traditional folk medicine obtained from the plant resources. An attempt has been made to evaluate the antimicrobial activity of the medicinal plant- Ocimum sanctum based on prevalent diseases and ethnobotanical knowledge, against Aspergillus brasiliensis. In order to assess the antifungal efficacy of Tulsi, the agar well diffusion method was employed. A Soxhlet apparatus was used to prepare the extraction of Tulsi leaves. Five different concentrations (0.2mg/ml, 0.4mg/ml, 0.6mg/ml, 0.8mg/ml, 1.0mg/ml) of tulsi extract were prepared by employing aqueous solution as well as various solvents such as acetone, chloroform, and methanol. The extracts were subjected to microbiological investigation to evaluate antimicrobial properties of tusli. ZOI were observed in mm. At a concentration of 1.0mg/ml, chloroform displayed the maximum zone of inhibition (21mm) among the various solvents tested. The leaf extracts obtained from solvents (acetone, chloroform, and methanol) exhibited antifungal activity against A. brasiliensis. These findings highlight that extracts from O. sanctum represent valuable sources of natural bioactive compounds, with potential application as potent antimicrobial drugs for combating a range of pathogenic microorganisms.
Key words: Antifungal activity, O.sanctum, Zone of inhibition, A.brasiliensis. .