Original Research Article
Year: 2019 | Month: July | Volume: 9 | Issue: 7 | Pages: 12-18
The Role of 8-Hydroxy-2'-Deoxyguanosine and Total Antioxidant Capacity in Women with Preterm Birth: A Preliminary Study
Vinita Verma1*, Hina Oza2, Riddhi Thaker1*, Sunil Kumar1
1Divsion of Reproductive & Cyto-toxicology, ICMR-National Institute of Occupational Health,
Ahmedabad-380016, India
2Department of Obstetrics and Gynecology, Civil Hospital, Ahmedabad-380016, India
*Ph. D Scholar, Gujarat University, Navrangpura, Ahmedabad-380009, India
Corresponding Author: Sunil Kumar
ABSTRACT
Objective: Pregnancy is associated with increased oxidative stress, and exaggeration of oxidative damage is significant in occurrence of pregnancy complications. The preterm or premature births are linked at least in part with oxidative stress (OS) and DNA damage. Therefore, the study was conducted with the aim to assess the levels of 8-hydroxy-2'–deoxyguanosine (8-OHdG) and total antioxidant capacity (TAC) in the women with or without Preterm birth (PTB).
Material & Methods: A total of 77 women comprising full term (controls; n=39) and preterm (cases; n=38) birth were enrolled. Predesigned proforma were filled to collect demographic, personal, medical and reproductive history of the subjects. The concentrations of 8-OHdG and TAC were determined in serum of subjects by ELISA technique.
Results: A higher 8-OHdG concentrations were found in the serum of women with PTB compared to control group i.e. full-term birth while subjects with PTB had slightly lower level of TAC with respect to the control. Further, a significant negative correlation (p<0.05) between 8-OHdG and TAC levels was observed. This indicates that as the DNA damage increases, there is a decrease in total antioxidant capacity. Risk factors analysis by logistic regression demonstrated that the risk factors such as mental stress (OR 5.63, 95 CI1.35 to 23.47, p<0.05) and 8-OHdG (OR 1.14, 95 CI 1.00 to 1.30, p<0.05) were associated with preterm birth.
Conclusion: The data obtained so far reveals that the DNA damage marker (8-OHdG) and mental stress were might be associated with preterm birth.
Key words: Antioxidant capacity, DNA damage, Mental stress, Oxidative stress, Preterm birth.