Original Research Article
Year: 2019 | Month: May | Volume: 9 | Issue: 5 | Pages: 22-27
Histopathological Patterns of Testicular Biopsies in Azoospermic Infertile Males
Dr. Anita R Gune1, Dr. Ashalata D Patil2, Dr. Rahul P Gune3, Dr. Rajendra S Patil4
1Associate Professor, Department of Anatomy, D.Y. Patil Medical College, Kolhapur.
2Professor, Department of Anatomy, D.Y. Patil Medical College, Kolhapur.
3Consultant Urologist, Nirmal Nursing Home, Mirjakar Tikkti, Kolhapur.
4Associate Professor, Department of Pathology, D.Y. Patil Medical College, Kolhapur.
Corresponding Author: Dr. Anita R Gune
ABSTRACT
Introduction: Testicular biopsy is important in categorizing patients with Azoospermia and provides useful information and guidelines for further treatment. Histopathological findings of testicular biopsies are of significant importance in making decision for selection of cases for Intracytoplasmic sperm injection (ICSI) in patients with non-obstructive azoospermia.
Objective: To categorize histopathological patterns of testicular biopsy specimens from Azoospermic infertile males to Modified Johnson scoring system.
Methodology: Testicular biopsies from male patients with history of infertility and azoospermia were included in this study. All biopsies were received in 10% Bovian fixative and routinely processed and stained with Haematoxylin and Eosin (H and E). Clinical data was obtained and recorded on a proforma. All cases were examined microscopically and various histological patterns were evaluated and categorized according to the histopathological patterns and Modified Johnson scoring was performed.
Results: A total 32 cases of testicular biopsies from Azoospermic males were evaluated. Most frequent pattern found was of Seminiferous tubule hyalinization (34.375%), further categorized as Johnson score 1. The second most frequent was Germ cell maturation arrest (25%), further categorized as Johnson score 8-3.The third common was Sertoli cell only syndrome (18.75%), further categorized as Johnson score 2. There were 3 cases that showed mixed pattern and 2 case showed discordant pattern.
Conclusion: This study outlines the different histopathological patterns of testicular biopsies in cases of male infertility in our setup and identifies seminiferous tubule hyalinization as the most common pathological finding amongst various histopathological patterns.
Key words: Testicular biopsy, hypospermatogenesis, male infertility