Original Research Article
Year: 2018 | Month: August | Volume: 8 | Issue: 8 | Pages: 66-73
Speciation and Antibiotic Resistance Pattern of Coagulase Negative Staphylococci (CONS)-Need of Time
Dr. Abhishek Debnath1, Dr. Suvarna Sande (Tathe)2
1Resident, 2Professor & HOD, Dept. of Microbiology, Jawaharlal Nehru Medical College, Sawangi (Meghe), Wardha, Maharashtra
Corresponding Author: Dr. Suvarna Sande (Tathe)
Introduction: Coagulase negative staphylococci (CONS) are part of normal flora and recognized as important pathogen for immune compromised hosts, patients with medical devices and nosocomial infections.
Because of increasing clinical significance of CONS, accurate species identification of CONS and Antimicrobial susceptibility test (AST) to various classes of antimicrobials is highly desirable.
Aim and objectives: To identify species of CONS isolated from clinically significant samples and to study antibiotic susceptibility profile with reference to methicillin resistance and inducible and constitutive clindamycin resistance.
Material and Methods: 150 CONS strains isolated from clinically significant samples were identified by different conventional methods and AST was studied by Kirby-Bauer Disk Diffusion method.
Methicillin resistance and Inducible and constitutive clindamycin resistance (D test) was detected according to CLSI guidelines.
Results: Among 150 CONS strains isolated, predominant was S.epidermidis 50.66%, followed by S.hemolyticus 24%. AST revealed resistance to penicillin in 96%, erythromycin in 70 % (11.42% were inducible MLSᴃ phenotype and 4.76% constitutive MLSᴃ phenotype), ciprofloxacin in 67.33 % and cefoxitin in 65.33 % (MRCONS).
Conclusion: The increasing importance of CONS particularly as nosocomial and opportunistic pathogen and emergence of drug resistance demonstrates need for speciation and detection of resistance pattern in CONS, which is necessary for appropriate antibiotic treatment.
Key words: Coagulase negative staphylococci (CONS), Antimicrobial susceptibility test (AST).