Original Research Article
Year: 2018 | Month: May | Volume: 8 | Issue: 5 | Pages: 92-96
Biofilm Formation as an Uropathogenic Marker of E. Coli Isolates Causing Urinary Tract Infections among Pregnant Women
Smrutirekha Mishra1, Dr. Sukantibala Mohapatra2, Dr M R Ranjit3, Dr G K Panda4
1PhD Scholar, Utkal University, Vanivihar, Bhubaneswar.
2Associate Professor, Dept. of Microbiology, OUAT, Bhubaneswar.
3Scientist F, ICMR- Regional Medical Research Centre, Bhubaneswar-751023
4Associate Professor, Dept. of PTSR, MSM Institute of Ayurveda, BPSMV, Khanpur kalan, Sonipat, Haryana
Corresponding Author: Smrutirekha Mishra
ABSTRACT
Background: Urinary tract infection is a major health concern in human society and also represents one of the most frequent hospital-acquired infections. Prevalence of UTI among pregnant women is more due to the hormonal and physiological changes during pregnancy. One of the bacterium mostly incriminated in UTI include Escherichia coli having several uropathogenic markers those allow them to colonise and persist in the urogenital tract.
Objectives: The present research work was performed to,
- Study the occurrence of E.coli isolates causing UTI among pregnant women.
- Detect the presence of biofilm formation in E. coli isolates as an uropathogenic marker.
Materials and Methods: A total of 136 no. of E. coli isolates were recovered out of 300 urine samples collected from pregnant women suspected to having UTI. The E. coli isolates were screened for biofilm formation by the method described by O’Toole and Kolter. Hemolysin production was also tested on 5% sheep blood agar.
Results: Only 19 E coli isolates found to have biofilm formation as a pathogenic marker and hemolysin production was seen in 57 cases.
Key words: Urinary tract infection, Pregnancy, uropathogenic markers, Biofilm formation.