Original Research Article
Year: 2018 | Month: March | Volume: 8 | Issue: 3 | Pages: 53-59
Antibiotic Resistance Profile & β-Lactamase Production in Pseudomonas Aeruginosa
Mr. Pankaj Kaushik1, Dr. Silpi Basak2
1Intern, Jawaharlal Nehru Medical College Wardha (MS),
2Professor, Department of Microbiology, Jawaharlal Nehru Medical College Wardha (MS),
Corresponding Author: Dr. Silpi Basak
ABSTRACT
Background & objectives: In the recent years, antibiotic resistance in Pseudomonas aeruginosa is on the rise. It can develop resistance by mutation, by acquiring resistance genes or by producing newer β-lactamases like ESBLs, AmpC β-lactamases and MBLs. Hence, the study was aimed to detect the antibiotic resistance profile in P. aeruginosa and the incidence of newer β-lactamases producing strains.
Methods: The present cross-sectional study included 50 clinical isolates of P. aeruginosa. Antibiotic susceptibility test was done by Kirby-Bauer disc diffusion method and ESBLs, AmpC β-lactamases and MBLs were detected by phenotypic methods.
Results: Highest antibiotic resistance was observed with Cefoxitin (98% strains). 45 (90%) strains produced ESBLs, AmpC β-lactamases and MBLs either alone or in combination.
Interpretation & Conclusion: Phenotypic detection of newer β-lactamases should be done in Clinical Microbiology Laboratory.
Key words: Pseudomonas aeruginosa, Antibiotic resistance, newer β-lactamases.