Original Research Article
Year: 2018 | Month: February | Volume: 8 | Issue: 2 | Pages: 199-207
Effect of Phyllanthus Amarus Leaf Extract on the Serum Lipid Profile of Alloxan-Induced Diabetic Albino Wistar Rats in College of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria
Ezeugwunne IP1,2, Chukwuma FC2, Ogbodo EC3, Okpogba AN1, Analike RA4, Okwara JE4, Mbaeri TU5, Amah AK6.
1Department of Human Biochemistry, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi Campus;
2Department of Environmental Health Science, Nnamdi Azikiwe University, Nnewi Campus;
3Department of Medical Laboratory Science, Faculty of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus;
4Department of Chemical Pathology, Faculty of Medicine, Nnamdi Azikiwe University, Nnewi Campus,
5Department of Surgery, Faculty of Medicine, Nnamdi Azikiwe University, Nnewi Campus;
6Department of Physiology, College of Medicine, Imo State University, Owerri.
Corresponding Author: Ezeugwunne IP
ABSTRACT
This study was designed to investigate the effect of Phyllanthus amarus (PA) leaf extract on serum lipid profile in alloxan induced diabetic albino wistar rats. A total of 30 albino wistar rats each weighing 100g were assembled and divided into 3 groups (A-C) consisting of 10 rats. Group A received PA treatment, B was without PA treatment while group C served as the control group. 400mg/kg of aqueous extract of PA leaf was administered orally to the rats in group A but not in group B while group C received only water for 7 days. Blood samples were collected into plain containers for estimation of biochemical parameters (TC, TG, LDL and HDL) respectively. Serum TC, TG, LDL and HDL were analyzed using standard methods respectively. There was a significant decrease in the mean serum level of TC (2.10±0.11 vs 2.6±0.16; p=0.000), TG (0.55±0.03 vs 0.61±0.04; p=0.000) and LDL (1.07±0.03 vs 1.53±0.06; p=0.000) whereas the mean serum level of HDL was significantly raised after PA administration (0.77±0.09 vs 0.72±0.06; p=0.000). Again, the result shows a significant decrease in the mean weight of the subjects after PA administration (98.80±1.03 s 119.40±1.17; p= 0.000). This study revealed hypolipidemic as well as anti-obesity effects of PA use. Therefore, PA use could be of importance in prevention and management of cardiovascular diseases and obesity in patients.
Key words: Phyllanthus amarus, Low density lipoprotein, High density lipoprotein, Triglyceride, Total cholesterol, wistar albino rat.