Original Research Article
Year: 2018 | Month: October | Volume: 8 | Issue: 10 | Pages: 145-148
In Vitro Antimicrobial and Phytochemical Study of Plant Extract
Upadhyay P S
Assistant Professor, Kaumarbhritya Department, Faculty of Ayurveda, IMS BHU, Varanasi, UP, India
Background and Aim: Micro-organisms have developed resistance to many antibiotics and this have created immense clinical problem in the treatment of infectious disease. This resistance has increased due to indiscriminate use of commercial anti-microbial drugs commonly used in the treatment of infectious disease. Present study was conducted to investigate antibacterial properties as well as phytochemical study of extract of the Musta, Pippali, Ativisha and Karkatshringi.
Material and method- Methanolic extract of each drug was collected in separate sterile vial and preserved at temperature 40C. Kirby-Bauer method was used as suggested by NCCLS (National Committee for Clinical Laboratory Services), USA, 2000 for antimicrobial study. For phytochemical study the dried samples were re-suspended in HPLC grade Methanol for HPLC analysis. Result- Six phenolic acids were detected in the four plant samples. The MIC of active extracts was determined by tube dilution method. After incubation the inhibition was observed on Escherichia coli ATCC 25992, V. cholerae 01 Classical and Enterococcus faecalis. Escherichia coli ATCC 25992 was sensitive to alcoholic as well as aqueous extract of Pippali.
Conclusion:- it means these plant extractshaving high antioxidant property and antibacterial effect as well.
Key words: Plant extract, Micro-organisms, Anti-microbial, Phytochemical.