Original Research Article
Year: 2016 | Month: December | Volume: 6 | Issue: 12 | Pages: 259-264
Dynamics Changes of Proteolytic Balance in Blood Plasma under Experimental Chemical Burns of Esophageal Development in Rats
Ishchuk T.V., Koval T.V., Savchuk O.M., Raetska Ya.B., Ostapchenko L.I.
Taras Shevchenko National University of Kyiv, ESC “The Institute of Biology”, Ukraine.
Corresponding Author: Ishchuk T.V.
ABSTRACT
Background: Proteinases control all metabolic processes involved in neurotransmitter receptor signalling in coordinating the process of fibrinolysis, hemocoagulation, activation of the complement system. In critical conditions there is an infringement of dynamic equilibrium between the proteases and their inhibitors, which is an important link in the pathogenesis of many diseases. In literature there is no data on patterns of change proteases and their inhibitors in the bloodstream after esophagus burning injuries. These experimental data would allow to assess the nature of reparation process in case of esophagus burning, and accordingly develop effective methods of proteolysis targeted correction.
Aim: To investigate features of proteolytic enzymes system and their inhibitors in the pathogenesis post burning process.
Methods: Chemical burns in animals were experimentally modeled in the following way: alkaline esophageal burn was caused by 20% NaOH and acid esophageal burn was caused by 30% CCl3COOH. The total proteolytic activity analyzed by method of caseinolytic activity with modifications. The level of α1-AT and α2-MG were determined using the method Nartykova.
Results: The result of the research it's shown that the development of alkali burn there was observed increased activity of MMP and serine proteases on 15th and 21st day against a background of increased levels of α2-MG. However when modelling acid burn, decrease of proteolytic enzymes on 15th and 21st day and increased α1-AT level during experiment was observed.
Conclusion: As a result of the research there were showed different dynamics of changes in alkaline and acid burns of the esophagus. Thus, the results may indicate inflammation at the latest experiment terms in modelling the esophagus alkali burns.
Key words: esophagus burn, MMP, serine protease, protease inhibitor.