Year: 2023 | Month: November | Volume: 13 | Issue: 11 | Pages: 304-309
DOI: https://doi.org/10.52403/ijhsr.20231136
Plasma Amino Acids Profile: Established Reference Range in Indian Children
Komal Uppal1, Richa Nijhawan2, Sunil Kumar Polipalli3, Sanjeev Kumar Pandey4, Avinash Lomash5, Somesh Kumar6, Ankur Jindal7, Seema Kapoor8
1,2,8Paediatrician, 3,5,6,7Scientist, 4Assistant Professor
1,2,3,5,6,7,8Division of Genetics and Metabolism, Department of Pediatrics, Maulana Azad Medical College (Delhi University), Delhi 110002
4Hind Institute of Medical Sciences, Uttar Pradesh
5Medanta - The Medicity, CH Baktawar Singh Rd, Medicity, Islampur Colony, Sector 38, Gurugram, Haryana 122001
Corresponding Author: Sunil Kumar Polipalli
ABSTRACT
Reference values were determined for 17 plasma free amino acids from measurements done in 248 healthy children ranging from 0 to 12 years of age. The method used is based on precolumn derivatization of primary amino acids with o-phthalaldehyde (OPA) and 9-fluorenylmethy[l] chloroformate (FMOC) for secondary amino acids, separation of the derivatives by reversed-phase chromatography, and quantification by fluorescence detection. We propose to present the age-specific distribution of plasma amino acid concentrations. Complete separation was achieved within 18 min. Total analysis time, including derivatization, chromatography, and reequilibration of the column, was 25 min. The assay was linear from 0 to 518 umol/L for all amino acids. Recovery of amino acids added to plasma samples was 92-104%. Within-run precision (CV) was 0.21-6.41% and between- run precision was 3.34-15.75%. This way of presenting amino acid concentrations may facilitate the follow-up of patients with inborn errors of amino acid metabolism.
Key words: Free Amino Acids, Reversed-phase chromatography.