Original Research Article
Year: 2022 | Month: August | Volume: 12 | Issue: 8 | Pages: 222-229
DOI: https://doi.org/10.52403/ijhsr.20220830
To Evaluate Antimycobacterial Activity of Rasamanikya by Using Advance Bact/ALERT 3-D Automated Liquid Culture WSR to Tuberculosis
Dr. Yogesh Madgulwar1, Dr. Vinod Ramteke2, Dr. Raman Belge3
1P.G. Scholar, Dept. of Rasashastra & Bhaishajya Kalpana, Shri Ayurved Mahavidyalaya,Nagpur
2P.G. Guide, Associate Professor Dept. of Rasashastra & Bhaishajya Kalpana, Shri Ayurved Mahavidyalaya, Nagpur
3Professor & H.O.D. Dept. of Rasashastra & Bhaishajya Kalpana, Shri Ayurved Mahavidyalaya, Nagpur
Corresponding Author: Dr. Yogesh Madgulwar
ABSTRACT
Tuberculosis is respiratory disease. Tuberculosis generally affects the lungs, but it can also affect other parts of the body. Latent tuberculosis in which case no symptoms seen. Around 10% of latent infections progress to active disease which, if left untreated, kill about half of those affected. Typical symptoms of active TB are chronic cough with blood containing mucus,fever, night sweats and weight loss. Tuberculosis spread from one person to another person when people who have active TB in their lungs cough, spit, speak or sneeze. People with latent TB dont spread the disease. There is no direct reference of tuberculosis in ayurveda but we could consider it under Rajaykshma by its signs and symptoms. Rajaykshma is group of disease which includes vitiation of tridosha and Sapta dhatu, due to indulging in Sahasa, Vega Sandarana, Kshaya, Vishmashana, there is manifestation of Ekadasha Rupa. Rasamanikya is a herbo-mineral formulation indicated for Rajaykshma. Rasamanikya is a Kupipakwa Rasayan. It was prepared according to ref. of Brihatrasarajasundar mentioned in Rajaykshmarogadhikar. Hence it was chosen to evaluate antimycobacterial activity by using Bact alert 3 D automated liquid culture.
Aim: To evaluate antimycobacterial activity of Rasamanikya by using advance Bact/ALERT 3-D Automated liquid culture WSR to Tuberculosis.
Objective:
To prepare Rasamanikya
To have analytical test of Rasamanikya and to evaluate antimycobacterial activity by using Bact alert 3-D automated liquid culture.
Material And Methods: Rasamanikya was prepared according to ref mentioned in Brihatrasarajasundar. Analytical tests of Rasamanikya were performed according to methods described in ayurvedic pharmacopoeia of india and antimycobacterial activity of Rasamanikya was carried out with the help of Bact alert 3-D automated liquid culture.
Results: Rasamanikya extract at MIC 1g/ml was able to achieve significant (P < 0.001 vs MTB culture & Other concentration of herb) antimycobacterial activity which was comparable to MIC of TB drugs. Another concentration of Rasamanikya although showed antimycobacterial activity based on delayed growth time compared to MTB culture however, they are not significant compared to TB drugs.
Key words: Tuberculosis, Antimycobacterial activity, Bact alert 3-D automated liquid culture system, Rajayakshma, Rasamanikya.