Original Research Article
Year: 2022 | Month: July | Volume: 12 | Issue: 7 | Pages: 163-171
DOI: https://doi.org/10.52403/ijhsr.20220724
Comparative Analysis of Thinprep and Cellsolutions Liquid-Based Cervical Cytology along with Human Papillomavirus DNA Testing: A Study of 412 Cases
Kartiki A Jadhav, Shaikhali M Barodawala, Ashwini Patkar, Kunjal Lila, Pooja Sarang, Rakhi Bajpai Dixit, Kirti Chadha
Department of Surgical Pathology and Molecular Biology, Global Reference Laboratory, Metropolis Healthcare Limited, Vidyavihar, Mumbai, Maharashtra, India 400070
Corresponding Author: Dr. Kirti Chadha, kirti.chadha@metropolisindia.com
ABSTRACT
Objective: The objective of the study was to compare the effectiveness and feasibility of current cervical cancer screening strategies i.e., CellSolutions and ThinPrep liquid-based cytology (LBC) along with Human Papillomavirus based (HPV) DNA testing in the Indian population for accurate and early detection of cervical cancer.
Methods: ThinPrep (206) and CellSolutions (206) based total of 412 LBC samples were studied, out of which 307 were also used for HPV co-testing. HPV-based DNA testing with hybrid capture 2 followed by PCR was carried out to identify high and low-risk genotypes. The precancerous lesions were reported according to the revised Bethesda classification.
Results: ThinPrep and CellSolutions-based LBC with HPV co-testing showed a significant decrease in the incidence rate of cervical cancer. The detection rate of abnormal smears was 3.88, 2.91, and 10.4% in ThinPrep, CellSolutions, and HPV testing, respectively. Low-grade squamous intraepithelial lesion (LSIL) was the most common abnormality compared to High-grade SILs in both the LBC techniques. The most common high-risk HPV genotypes detected were 16, 18, 56, and 66, while low-risk were 6, 42, 53, 62, 81, and 30, respectively.
Conclusions: Cervical cancer screening strategies evaluated in the Indian population. CellSolutions is comparable to ThinPrep, HPV has the highest detection rate of abnormal smears as compared to LBCs. HPV along with LBC co-testing improves precision, early detection and eliminates unnecessary colposcopy procedures.
Key words: Liquid-based cytology, Cervical cancer, ThinPrep, CellSolutions, HPV.