Original Research Article
Year: 2022 | Month: February | Volume: 12 | Issue: 2 | Pages: 16-25
DOI: https://doi.org/10.52403/ijhsr.20220203
Characterization and Antibiotic Susceptibility of Bacteria Isolated from Clinical Specimens in Rajshahi, Bangladesh
Shekha Nita Mondal1, Md. Ariful Islam1,2, Tamanna Zerin1
1Department of Microbiology, Stamford University Bangladesh, 51, Siddeswari Road, Dhaka 1217, Bangladesh.
2Rajshahi Metropolitan Hospital Ltd. and Diagnostic Center, C & B Mor, Laxmipur, Kajihata, Rajpara, Rajshahi 6000, Bangladesh
Corresponding Author: Tamanna Zerin
ABSTRACT
Infectious diseases caused by antibiotic-resistant infectious bacteria are a major threat to human survival and development. This is a retrospective study for a one-year period from January 2020 to December 2020 with a total of 1,112 urine, 318 blood, 167 stool, and 72 pus samples conducted in the Microbiology Laboratory of Rajshahi Metropolitan Hospital Ltd. and Diagnostic Center, Rajshahi, Bangladesh. Bacterial isolation, characterization, and antibiotic susceptibility were performed following the standard microbiological methods. Out of 1112 urine, 318 blood, 167 stool, and 72 pus specimens, bacteria were detected in 345 urine, 86 blood, 44 stool, and 50 pus specimens, respectively. Specimens from females (57.10%) were found more positive than those from males (42.90%) only in the case of urine samples. Among all the specimens, the highest number of infections was recorded in the age group 31–40, except in stool, where the highest positive cases were evidenced in the 0–10 age group. The greatest number of specimens was received, mostly in the summer and rainy seasons. However, E. coli bacteria were the most common among all the specimens excluding pus, where Staphylococcus aureus was the highest in number. Gram-positive and gram-negative bacteria showed higher sensitivity towards Cefepime (against pathogens of blood and stool samples) along with Imipenem, and Meropenem (pathogens of both urine and pus samples). A terrifying scenario was the high degree of resistance of those isolated bacteria towards the 3rd and 4th generation cephalosporin antibiotics. This study might be helpful for physicians to rationalize empiric treatment strategies and provide awareness among government authorities and the population.
Key words: Bangladesh, Clinical specimens, Resistance, Retrospective, Sensitivity.