Original Research Article
Year: 2021 | Month: May | Volume: 11 | Issue: 5 | Pages: 362-378
DOI: https://doi.org/10.52403/ijhsr.20210557
Comparison of in Vitro Vasculogenesis Potential between ASCs with BM-MSCs
Ani Oranda Panjaitan1, Dewi Sukmawati2, Ria Anggraeni3, Jeanne Adiwinata Pawitan4
1Departement of Anatomy and Histology Faculty of Medicine, Universitas Kristen Indonesia, Jakarta
2Departement of Histology, Faculty of Medicine, Universitas Indonesia
3Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine Universitas Indonesia, Indonesia
4Stem Cell Medical Technology Integrated Service Unit, Dr Cipto Mangunkusumo General Hospital/Faculty of Medicine Universitas Indonesia, Indonesia
Corresponding Author: Ani Oranda Panjaitan
ABSTRACT
Tube formation assay is the most widely used method as a vasculogenesis/ angiogenesis test in vitro. Mesenchymal stem cells (MSCs) are multipotent adult cells. The paracrine effect of MSCs on neovascularization is well known. In general, MSCs do not express CD34 hematopoietic surface marker, but according to some experts, bone marrow mesenchymal stem cells (BM-MSCs) express CD34 in vivo and lose their expression when they are cultured in vitro, while adipose-derived stem cells (ASCs) still have CD34 expression in the early passages when cultured in vitro. BM-MSCs are the most widely used MSC, but ASCs are also used in stem cell therapy and tissue engineering for angiogenesis purposes. Until now, the potential of vasculogenesis between ASCs and BM-MSCs is still unclear. Expression of CD34 is also unknown whether affecting the quality of tube formation. This study wanted to compare the potential of vasculogenesis between ASC and BM-MSCs through tube formation test and CD34 expression.Measurements of vasculogenesis quality showed higher tube length, number of loopsand mean number of branch points on BM-MSC. Both BM-MSCs and ASCs showed low CD34 levels.BM-MSCs showed better tube formation ability compared with ASCs. No association was found between CD34 levels and MSC vasculogenesis capability.
Key words: ASCs, BM-MSCs, CD34, matrigel, tube formation.