Original Research Article
Year: 2020 | Month: June | Volume: 10 | Issue: 6 | Pages: 36-45
Gandhaka Rasayana: Possible Role as a Modulator of Fibroblast Cell Function in Wound Healing
Premalatha Shetty1, Prashanth Kedukodi2, Sahana Kamath2, Vishwanatha Udupi1
1Scientist, Department of Biotechnology & Microbiology, Sri Dharmasthala Manjunatheshwara Centre for Research in Ayurveda and Allied Sciences, Udupi-574118, Karnataka, India;
2Associate Professor, 3Assistant Professor, Department of Post Graduate studies in Shalyatantra,
4Research Officer, Department of Biotechnology & Microbiology,
Sri Dharmasthala Manjunatheshwara College of Ayurveda and Hospital, Udupi-574118, Karnataka, India;
Corresponding Author: Premalatha Shetty
ABSTRACT
Background & Objective: Gandhaka Rasayana (GR), a sulfur based ayurvedic formulation is used extensively in treatment of various skin and gastro-intestinal disorders and in healing of chronic non healing wounds. The rasayana is believed to play a significant role in repairing or rejuvenating of cellular functions. In this study, we investigated the effect of GR on fibroblast function.
Materials & Methods: Conditioned media were obtained from 3T3-L1 and McCoy fibroblast cells treated with GR extract. In-vitro studies were carried out to study the effect of conditioned medium on wound closure and expression of metallothioneins (MTs) and tissue inhibitor of metalloproteases (TIMP 1) in A549 cells.
Results and Conclusion: GR extract was found to induce increased expression of MTs to an extent of 1.5-1.9 fold in A549 cells. Under the experimental conditions employed, the conditioned media from GR treated McCoy and 3T3 were found to upregulate expression of MTs to an extent of 1.6-2.2 and 1.5 fold respectively in A549 cells. The conditioned medium from McCoy upregulated expression of TIMP 1 in A549 cells to a limited extent of around 1.35 folds. GR appears to affect fibroblast activation and modulate the expression of proteins involved in tissue remodeling. Conditioned media from both the fibroblast cell lines facilitated gap closure in A549 cell layer.
Key words: Gandhaka rasayana, Wound healing, Metallothioneins, Conditioned medium, Fibroblast