Original Research Article
Year: 2020 | Month: February | Volume: 10 | Issue: 2 | Pages: 32-36
Efficacy of Duku Seed Extract on Reducing Levels of HbA1c in Diabetic Model Rat
Wahyu Irawan NST1, Muhammad Totong Kamaluddin2, Theodorus3
1Medical Student of Medical Faculty of Sriwijaya University
2Senior Lecturer in Department of Pharmacology, Medical Faculty of Sriwijaya University
3Jl. Dr. Mohamm ad Ali RSMH Palembang KM Complex. 3.5, Palembang, 30126, Indonesia
Corresponding Author: Wahyu Irawan NST
ABSTRACT
Background: Diabetes mellitus (DM) is a multisystem metabolic disease due to abnormal insulin secretion, work and insulin function, or both. Abnormalities in the secretion or work of insulin cause abnormalities in the metabolism of carbohydrates, fats and proteins. Phytochemical test of duku seeds showed that all extract fractions contained flavonoids and terpenoids, whereas acetone and residual fractions contained alkaloids and saponins. Most plants that contain bioactive compounds such as flavonoids, saponoid, saponins and triterpenoids have antioxidant activity and other mechanisms as substances that have the potential to cause hypoglycemic effects. The goal of this research was to determine the efficacy of duku seed extract (Lansium domesticum Corr) on decreasing HbA1c levels.
Method: In vivo, pre-and post-test design has been conduction at Animal House Faculty of Medicine, Sriwijaya University from November to December 2019. Twenty-four white rats weighing between 150-200g divided into 4 groups. Rats were acclimated for 7 days, after acclimation rats were induced with alloxan 160 mg / kg intraperitoneally. The first, second, third group were given duku seed extract 50, 100, and 150 mg / kg of duku seed extract, and the fourth group was given glimepiride 0.018ng / kg / day. All treatments were given orally for one week. The data obtained were analyzed by normality test, paired t test, ANOVA, and Post Hoc test by using STATA.
Results: The rats were homogen on weight and HbA1C. Treatment of 50 and 100 mg / kg duku seed extract in rats showed a significant difference (p = 0.05) on day 7. Blood HbA1C levels decreased by 14.87% in duku extract 50mg / kgbb, while in seed extract 100 mg / kg body weight HbA1C levels reduced by 9.45%.
Conclusion: The administration of duku seed extract (Lansium domesticum) in diabetic rats can reduce blood HbA1C levels.
Key words: Duku seeds (L. Domesticum), alloxan, HbA1C, Diabetes Mellitus, in vivo study